SrasV1, Main, Exploration, bibRecord, 005064

Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells

Identifieur interne : 005064 ( Main/Exploration ); précédent : 005063; suivant : 005065

Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells

Auteurs : Ling Yi ; Zhengquan Li ; Kehu Yuan ; Xiuxia Qu ; Jian Chen ; Guangwen Wang ; Hong Zhang ; Hongpeng Luo ; Lili Zhu ; Pengfei Jiang ; Lirong Chen ; Yan Shen ; Min Luo ; Guoying Zuo ; Jianhe Hu ; Deliang Duan ; Yuchun Nie ; Xuanling Shi ; Wei Wang ; Yang Han ; Taisheng Li ; Yuqing Liu ; Mingxiao Ding ; Hongkui Deng ; Xiaojie Xu

Source :

Journal of Virology [ 0022-538X ] ; 2004.

RBID : PMC:521800

Descripteurs français

KwdFr :
- Animaux, Antiviraux (), Antiviraux (métabolisme), Antiviraux (pharmacologie), Cellules Vero, Chine, Chromatographie d'affinité, Flavonoïdes (pharmacologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Luciferases (génétique), Luciferases (métabolisme), Lutéoline, Plantes médicinales (), Plantes médicinales (métabolisme), Protéines de l'enveloppe virale (métabolisme), Spectrométrie de masse, Tanins hydrolysables (), Tanins hydrolysables (pharmacologie), Tests de sensibilité microbienne, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme), Virus du SRAS (), Virus du SRAS (pathogénicité).
MESH :
- génétique : Luciferases, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- métabolisme : Antiviraux, Glycoprotéines membranaires, Luciferases, Plantes médicinales, Protéines de l'enveloppe virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- pathogénicité : Virus du SRAS.
- pharmacologie : Antiviraux, Flavonoïdes, Tanins hydrolysables.
- Animaux, Antiviraux, Cellules Vero, Chine, Chromatographie d'affinité, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Lutéoline, Plantes médicinales, Spectrométrie de masse, Tanins hydrolysables, Tests de sensibilité microbienne, Virus du SRAS.
Wicri :
- geographic : République populaire de Chine.

English descriptors

KwdEn :
- Animals, Antiviral Agents (chemistry), Antiviral Agents (metabolism), Antiviral Agents (pharmacology), Cell Line, China, Chlorocebus aethiops, Chromatography, Affinity, Flavonoids (pharmacology), HIV-1 (genetics), HIV-1 (metabolism), Humans, Hydrolyzable Tannins (chemistry), Hydrolyzable Tannins (pharmacology), Luciferases (genetics), Luciferases (metabolism), Luteolin, Mass Spectrometry, Membrane Glycoproteins (metabolism), Microbial Sensitivity Tests, Plants, Medicinal (chemistry), Plants, Medicinal (metabolism), SARS Virus (drug effects), SARS Virus (pathogenicity), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (metabolism).
MESH :
- chemical , chemistry : Antiviral Agents, Hydrolyzable Tannins.
- chemical , genetics : Luciferases.
- chemical , metabolism : Antiviral Agents, Luciferases, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , pharmacology : Antiviral Agents, Flavonoids, Hydrolyzable Tannins.
- geographic : China, Luteolin, Spike Glycoprotein, Coronavirus.
- chemistry : Plants, Medicinal.
- drug effects : SARS Virus.
- genetics : HIV-1.
- metabolism : HIV-1, Plants, Medicinal.
- pathogenicity : SARS Virus.
- Animals, Cell Line, Chlorocebus aethiops, Chromatography, Affinity, Humans, Mass Spectrometry, Microbial Sensitivity Tests, Vero Cells.

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-β-d-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 μM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521800

DOI: 10.1128/JVI.78.20.11334-11339.2004
PubMed: 15452254
PubMed Central: 521800

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells</title>
<author><name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Zhengquan" sort="Li, Zhengquan" uniqKey="Li Z" first="Zhengquan" last="Li">Zhengquan Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yuan, Kehu" sort="Yuan, Kehu" uniqKey="Yuan K" first="Kehu" last="Yuan">Kehu Yuan</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Luo, Hongpeng" sort="Luo, Hongpeng" uniqKey="Luo H" first="Hongpeng" last="Luo">Hongpeng Luo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhu, Lili" sort="Zhu, Lili" uniqKey="Zhu L" first="Lili" last="Zhu">Lili Zhu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Lirong" sort="Chen, Lirong" uniqKey="Chen L" first="Lirong" last="Chen">Lirong Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zuo, Guoying" sort="Zuo, Guoying" uniqKey="Zuo G" first="Guoying" last="Zuo">Guoying Zuo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duan, Deliang" sort="Duan, Deliang" uniqKey="Duan D" first="Deliang" last="Duan">Deliang Duan</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Yuqing" sort="Liu, Yuqing" uniqKey="Liu Y" first="Yuqing" last="Liu">Yuqing Liu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xu, Xiaojie" sort="Xu, Xiaojie" uniqKey="Xu X" first="Xiaojie" last="Xu">Xiaojie Xu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">15452254</idno>
<idno type="pmc">521800</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC521800</idno>
<idno type="RBID">PMC:521800</idno>
<idno type="doi">10.1128/JVI.78.20.11334-11339.2004</idno>
<date when="2004">2004</date>
<idno type="wicri:Area/Pmc/Corpus">000685</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000685</idno>
<idno type="wicri:Area/Pmc/Curation">000685</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000685</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001465</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001465</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:15452254</idno>
<idno type="wicri:Area/PubMed/Corpus">002B38</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B38</idno>
<idno type="wicri:Area/PubMed/Curation">002B38</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B38</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002A21</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002A21</idno>
<idno type="wicri:Area/Ncbi/Merge">000A90</idno>
<idno type="wicri:Area/Ncbi/Curation">000A90</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000A90</idno>
<idno type="wicri:doubleKey">0022-538X:2004:Yi L:small:molecules:blocking</idno>
<idno type="wicri:Area/Main/Merge">005430</idno>
<idno type="wicri:Area/Main/Curation">005064</idno>
<idno type="wicri:Area/Main/Exploration">005064</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells</title>
<author><name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Zhengquan" sort="Li, Zhengquan" uniqKey="Li Z" first="Zhengquan" last="Li">Zhengquan Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yuan, Kehu" sort="Yuan, Kehu" uniqKey="Yuan K" first="Kehu" last="Yuan">Kehu Yuan</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Luo, Hongpeng" sort="Luo, Hongpeng" uniqKey="Luo H" first="Hongpeng" last="Luo">Hongpeng Luo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhu, Lili" sort="Zhu, Lili" uniqKey="Zhu L" first="Lili" last="Zhu">Lili Zhu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Lirong" sort="Chen, Lirong" uniqKey="Chen L" first="Lirong" last="Chen">Lirong Chen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zuo, Guoying" sort="Zuo, Guoying" uniqKey="Zuo G" first="Guoying" last="Zuo">Guoying Zuo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Duan, Deliang" sort="Duan, Deliang" uniqKey="Duan D" first="Deliang" last="Duan">Deliang Duan</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Liu, Yuqing" sort="Liu, Yuqing" uniqKey="Liu Y" first="Yuqing" last="Liu">Yuqing Liu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xu, Xiaojie" sort="Xu, Xiaojie" uniqKey="Xu X" first="Xiaojie" last="Xu">Xiaojie Xu</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of Virology</title>
<idno type="ISSN">0022-538X</idno>
<idno type="eISSN">1098-5514</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antiviral Agents (chemistry)</term>
<term>Antiviral Agents (metabolism)</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Cell Line</term>
<term>China</term>
<term>Chlorocebus aethiops</term>
<term>Chromatography, Affinity</term>
<term>Flavonoids (pharmacology)</term>
<term>HIV-1 (genetics)</term>
<term>HIV-1 (metabolism)</term>
<term>Humans</term>
<term>Hydrolyzable Tannins (chemistry)</term>
<term>Hydrolyzable Tannins (pharmacology)</term>
<term>Luciferases (genetics)</term>
<term>Luciferases (metabolism)</term>
<term>Luteolin</term>
<term>Mass Spectrometry</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Microbial Sensitivity Tests</term>
<term>Plants, Medicinal (chemistry)</term>
<term>Plants, Medicinal (metabolism)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (pathogenicity)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Antiviraux ()</term>
<term>Antiviraux (métabolisme)</term>
<term>Antiviraux (pharmacologie)</term>
<term>Cellules Vero</term>
<term>Chine</term>
<term>Chromatographie d'affinité</term>
<term>Flavonoïdes (pharmacologie)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Luciferases (génétique)</term>
<term>Luciferases (métabolisme)</term>
<term>Lutéoline</term>
<term>Plantes médicinales ()</term>
<term>Plantes médicinales (métabolisme)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Spectrométrie de masse</term>
<term>Tanins hydrolysables ()</term>
<term>Tanins hydrolysables (pharmacologie)</term>
<term>Tests de sensibilité microbienne</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (métabolisme)</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term>
<term>Hydrolyzable Tannins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Luciferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antiviral Agents</term>
<term>Luciferases</term>
<term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Flavonoids</term>
<term>Hydrolyzable Tannins</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term>
<term>Luteolin</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants, Medicinal</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Luciferases</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HIV-1</term>
<term>Plants, Medicinal</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antiviraux</term>
<term>Glycoprotéines membranaires</term>
<term>Luciferases</term>
<term>Plantes médicinales</term>
<term>Protéines de l'enveloppe virale</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term>
<term>Flavonoïdes</term>
<term>Tanins hydrolysables</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Chromatography, Affinity</term>
<term>Humans</term>
<term>Mass Spectrometry</term>
<term>Microbial Sensitivity Tests</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Antiviraux</term>
<term>Cellules Vero</term>
<term>Chine</term>
<term>Chromatographie d'affinité</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Lutéoline</term>
<term>Plantes médicinales</term>
<term>Spectrométrie de masse</term>
<term>Tanins hydrolysables</term>
<term>Tests de sensibilité microbienne</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-<italic>O</italic>
-galloyl-β-<sc>d</sc>
-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 μM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.</p>
</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<name sortKey="Chen, Lirong" sort="Chen, Lirong" uniqKey="Chen L" first="Lirong" last="Chen">Lirong Chen</name>
<name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name>
<name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name>
<name sortKey="Duan, Deliang" sort="Duan, Deliang" uniqKey="Duan D" first="Deliang" last="Duan">Deliang Duan</name>
<name sortKey="Han, Yang" sort="Han, Yang" uniqKey="Han Y" first="Yang" last="Han">Yang Han</name>
<name sortKey="Hu, Jianhe" sort="Hu, Jianhe" uniqKey="Hu J" first="Jianhe" last="Hu">Jianhe Hu</name>
<name sortKey="Jiang, Pengfei" sort="Jiang, Pengfei" uniqKey="Jiang P" first="Pengfei" last="Jiang">Pengfei Jiang</name>
<name sortKey="Li, Taisheng" sort="Li, Taisheng" uniqKey="Li T" first="Taisheng" last="Li">Taisheng Li</name>
<name sortKey="Li, Zhengquan" sort="Li, Zhengquan" uniqKey="Li Z" first="Zhengquan" last="Li">Zhengquan Li</name>
<name sortKey="Liu, Yuqing" sort="Liu, Yuqing" uniqKey="Liu Y" first="Yuqing" last="Liu">Yuqing Liu</name>
<name sortKey="Luo, Hongpeng" sort="Luo, Hongpeng" uniqKey="Luo H" first="Hongpeng" last="Luo">Hongpeng Luo</name>
<name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name>
<name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name>
<name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name>
<name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name>
<name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name>
<name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name>
<name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name>
<name sortKey="Xu, Xiaojie" sort="Xu, Xiaojie" uniqKey="Xu X" first="Xiaojie" last="Xu">Xiaojie Xu</name>
<name sortKey="Yi, Ling" sort="Yi, Ling" uniqKey="Yi L" first="Ling" last="Yi">Ling Yi</name>
<name sortKey="Yuan, Kehu" sort="Yuan, Kehu" uniqKey="Yuan K" first="Kehu" last="Yuan">Kehu Yuan</name>
<name sortKey="Zhang, Hong" sort="Zhang, Hong" uniqKey="Zhang H" first="Hong" last="Zhang">Hong Zhang</name>
<name sortKey="Zhu, Lili" sort="Zhu, Lili" uniqKey="Zhu L" first="Lili" last="Zhu">Lili Zhu</name>
<name sortKey="Zuo, Guoying" sort="Zuo, Guoying" uniqKey="Zuo G" first="Guoying" last="Zuo">Guoying Zuo</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005064 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005064 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:521800
   |texte=   Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:15452254" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

Serveur d'exploration SRAS

Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells

Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.